Diversity of endophytic actinobacteria isolated from medicinal plants 859 the number of endophytic actinobacteria was dominant in the rhizome of k. The symbiotic relationship among endophytic fungi as well as plants gives endophytes the powerful capability to produce new bioactive substances 4,28. A total of 68 actinomycetes isolates were isolated from endophytic regions of healthy medicinal plants fig. Isolation and identification of endophytic fungi from medicinal plants and assessment of their antimicrobial activity. Medicinal plants are reported as host of some endophytic fungi that are involved in the coproduction of active metabolites alvin et al. Bioactivities of endophytic actinomycetes from selected medicinal plants in the world heritage site of saint katherine, egypt. Therefore, medicinal plants which are known to be used since. Among these isolates, only 12 isolates showed better chitinolytic potentials.
The choice of the plant to be used for exploring endophytes for bioactives is important. An endophyte is an endosymbiont, often a bacterium or fungus, that lives within a plant for at least part of its life cycle without causing apparent disease. Isolation of microbial endophytes from some ethnomedicinal plants of jammu and kashmir maroof ahmed, muzaffer hussain, manoj k. Frontiers a friendly relationship between endophytic fungi. It is worth mentioning that some endophytic microorganisms isolated from medicinal plants produce the same metabolites as their hosts, so if the bioactive product of the endophytic microorganisms is unique to it and is not produced by the host, this would not only reduce the need to harvest slow growing and possibly rare plants but also. Endophytic fungi cut off a large number of metabolites live. This study was conducted to determine taxonomical features and antimicrobial activities of 156 isolates of endophytic fungi collected from twigs of medicinal plants of lauraceae 67 isolates and rutaceae 89 isolates in central and northern taiwan.
Endophytic fungi of medicinal plants are currently being widely studied in the search for new potentially useful secondary metabolites. Here, the endophytic fungal communities associated with the leaves, stems, and roots of the four medicinal plant species were investigated by highthroughput sequencing technology. Medicinal plants and their endophytes are important resources for discovery of natural products. Pdf endophytic fungi residing in medicinal plants have the.
Endophytic bacteria have beneficial effect on plants such as plant growth promotion, supply of nutrients, less exposure to environmental stress and antagonistic control of plant pathogens hallmann et al. These isolated inhibit growing of pathogenic bacteria s. Endophytic fungi residing in medicinal plants have the ability to produce the same or similar pharmacologically active secondary metabolites as their hosts january 2017 hellenic plant protection. In the present investigation ten medicinal plants were analyzed for the presence of endophytic fungi.
Antimicrobial activity of crude extracts of endophytic. Pdf bioactivities of endophytic actinomycetes from. Lasparaginase is an important anticancer enzyme that is used in the first line treatment of acute lymphoblastic leukemia. Pdf identification of endophytic fungi of medicinal herbs. Despite the reports of ethnomedicinal plants of this region, the biodiversity and the endophytic microbes of this region remain unexplored. However, the natural resources of medicinal plants are gradually exhausted and access to plant bioactive compounds is challenged by the low levels at which. Extracellular enzymatic activity of endophytic fungal. Diversity of endophytic actinobacteria isolated from. Endophytic fungi residing in medicinal plants have the ability to produce the same or similar pharmacologically active secondary metabolites as their hosts a. Medicinal plants are the potential source of endophytic fungi with crucial natural products of pharmaceutical significance 6. Biocontrol potential of endophytic fungi in medicinal. Isolation of endophytic bacteria was done on tryptic soy agar media and characterized on the basis of morphological and biochemical characteristics. Endophytic fungal communities associated with leaves, stems. Screening of endophytic bacteria from anticancer medicinal.
Endophytic fungi or endophytes exist widely inside the healthy tissues of living plants, and are important components of plant microecosystems. Endophytic actinomycetes are one of the primary groups that share symbiotic relationships with medicinal plants and are key reservoir of biologically active compounds. A total of 81 thai medicinal plant species collected from forests were examined for the presence of endophytic fungi with biological activity. The compound was initially isolated from the pacific yew tree, taxus brevifolia wani et al. Endophytic fungi from medicinal plants article pdf available in economic botany 611. Endophytic fungi are microorganisms that colonize plant tissues intercellularly and. This study was conducted to isolate lasparaginaseproducing endophytic fungi from medicinal plants of family asteraceae. Special types of endophytic fungi of medicinal plants may be associated with the production of specific bioactive compounds needed by human. The 156 isolates of fungi were classified into 35 genera in 19 families based on morphological characteristics of mycelia and asexualsexual. Antimicrobial activity of endophytes from brazilian medicinal. Here, the endophytic fungal communities associated with the leaves, stems, and roots of the four medicinal plant species were investigated by.
A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. The aim of this study was to examine endophytic fungi in traditional chinese medicinal plants, and understand if these organisms have antimicrobial activities and they can be potentially used for biological control of plant diseases. Endophytes are an underinvestigated group of microorganisms that represent a plentiful and renewable source of bioactive and chemically new compounds with potential for exploitation in a wide variety of medical, agricultural, and industrial. Endophytic actinobacteria obtained from this work showed to have a. The samples were rinsed gently in running water to remove dust and debris. Exploration of endophytic microorganisms from selected. There is a number of ways by which endophytic bacteria can get access to a plants interior. Current identification and classification of actinobacteria are based a polyphasic approach, comprising morphological, physiological and molecular studies goodfellow et al.
Fungal enzymes are one of them which are used in food, beverages, confectionaries, textiles and leather industries to simplify the processing of raw materials. Research article endophytic fungal diversity in medicinal. Endophytic fungi from medicinal plants wiyakrutta et al. Antimicrobial biosynthetic potential and genetic diversity. These plants were selected based on its medicinal values such as antimicrobial, antitumor and antimutagenic properties.
Medicinal plant use can be traced to ancient agricultural societies, where indigenous populations have utilised them as therapies for many diseases davis. Endophytes are ubiquitous and have been found in all species of plants studied to date. Pdf endophytic fungi residing in medicinal plants have. Endophytic fungi are an excellent source of secondary metabolites as a natural product 3. Pdf bioactivities of endophytic actinomycetes from selected. Of the 68 isolates, the majority n 27, 3970% were isolated from roots followed by leaf n 24, 3529%, and stem n 17, 25% table s1. Species distribution and biological diversity of endophytic actinobacteria of medicinal plants are extensively influenced by ecological environment hou et al. Endophytic fungal communities associated with leaves. In a first study of ongoing research, endophytic bacteria were isolated from two medicinal plants, hypericum perforatum and ziziphora capitata with contrasting antimicrobial activities from the chatkal biosphere reserve of uzbekistan, and their plantspecific traits involved in biocontrol and. Endophytic bacteria with plant growth promotion and. Medicinal plants are known to harbor potential endophytic microbes, due to their bioactive compounds. Many medicinal plants possess antimicrobial activities, and have antagonistic endophytic fungi that help them protect from pathogen attack. A total of 25 fungal species were isolated from healthy leaves and identified based on the morphology of. Screening, isolation, biochemical and plant growth.
Five different plants were selected to enhance diversity as catharanthus roseus, oscimum sanctum, alovera, withania somnifera and murraya konengii. Isolation and identification of lasparaginaseproducing. Diversity and biopotential of endophytic actinomycetes. Fungal endophytes were isolated from healthy plant parts such as stem, root, rhizome, and inflorescence employing standard isolation methods. In this study, six selective medicinal plants were targeted for the first time for endophytic actinomycetes isolation from gibbon wild life sanctuary, assam, india, during winter. The sequencing of highly conserved macromolecules, notably 16s. To our knowledge, this is a first report on genetic diversity, antimicrobial evaluation and biosynthetic genes of endophytic actinomycetes associated with these medicinal plants of gibbon wild life sanctuary, assam. Production of bioactive secondary metabolites from. It is intriguing to notice that most of the work related to enzymatic profiling of endophytic fungi came from ethnobotanically important medicinal plants as a source of these fungi pavithra et.
Therefore, medicinal plants which are known to be used. In this study, we have investigated the diversity of endophytic actinomycetes associated with the six traditional medicinal plants. However, no information is available on whether such a relationship also exists in their endophytic fungal metabolites. The term endophytes includes a family of microorganisms that grow intraandor intercellularly in the tissues of higher plants without any symptoms on the plants in which they reside. Isolation and identification of endophytic fungi from. One of the most successful stories of natural products from endophytes is the multibilliondollar anticancer drug taxol paclitaxel. Antibacterial and antioxidant activities of endophytic. Endophytic fungi were isolated from endemic medicinal plants of tirumala hills by the procedure of standardized and modified method described by hallman et al. In this section we will follow their main colonization route from the rhizosphere and give a brief description of alternative ways of plant colonization by endophytes. Antibacterial and antioxidant activities of endophytic fungi extracts of medicinal plants from central sulawesi. Antibacterial and antioxidant activities of endophytic fungi. The analytical results indicate that the distribution andpopulation structureof endophytes canbe considerablyaffectedbyfactors, such as. The pr oduction of bioactive secondary metabolites by medicinal plants and by the endophytes pr ovided countless drugs selected as important therapeutic options for innumerabl e disease.
It also considersand their medicinal applications especially in the production of anticancer, antimicrobial, antioxidant, and. Antimicrobial biosynthetic potential and genetic diversity of. Since then, several other plants from the genus taxus have been reported to produce taxol. After which they were surface sterilized by submerging them in 75% ethanol for 2 min. Endophytic fungi from medicinal plants of virudhunagar district for antimicrobial activity 148 isolation of endophytic fungi leaves were thoroughly washed with mild detergent and running tap water and then airdried. Endophytic fungi residing in medicinal plants have the ability to. Pdf isolation and identification of endophytic fungi from. Papaver somniferum opium poppy is counted one of the most vital medicinal plant, belonging to family papaveraceae, which is a source of about 80 alkaloids of various classes that have pharmaceutical importance 7. Exploring the potential of endophytes from medicinal plants as.
Evaluation of antagonistic and plant growth promoting. The effects of endophytic fungi on the chemical composition of plants have been known by humans for centuries in the form of poisoning and disease as well as medicinal uses. Exploring the potential of endophytes from medicinal plants. Endophytes from medicinal plants and their potential for producing. The medicinal plant vitex negundo harbors diverse species of endophytic fungi. Diversity of endophytic actinobacteria in medicinal plants.
Endophytic fungal diversity in medicinal plants of western ghats. Endophytic fungi are ubiquitous organisms found in the plants, residing intercellular or intracellular, at least for a portion of their lives without causing apparent symptoms of infection. Frontiers antimicrobial activity of medicinal plants. Some of the endophytes were identified to genus or species level using. Almost all groups of microorganisms have been found in endophytic association with plants may it be fungi, bacteria or actinomycetes. Nov 21, 2012 endophytic fungi are ubiquitous organisms found in the plants, residing intercellular or intracellular, at least for a portion of their lives without causing apparent symptoms of infection. Endophytic fungi residing in medicinal plants have the. Pdf isolation and identification of endophytic fungi. Interestingly, genuine medicinal materials with the highest quality and best effects to a certain disease seems to have a special relationship with endophytic fungi. Jun 21, 2015 diversity of endophytic actinobacteria in medicinal plants. In this study, two bacterial endophytes from the medicinal plants echinacea purpurea and. Several previous studies have found a positive correlation between total antioxidant capacity tac and total phenolic content tpc of many medicinal plant extracts.
Microbially unexplored medicinal plants can have a genetically diverse microbial population with multifunctional plant growth promoting traits. Antimicrobial activity of endophytes from brazilian. Endophytic actinomycetes were most commonly recovered from roots 70% of all isolates followed by stems 17. Endophytic fungi exhibit a complex web of interactions with host plants and have been extensively studied over the last several years as prolific sources of new bioactive natural products. Biocontrol potential of endophytic fungi in medicinal plants. Three medicinal plants, aloe vera, mentha arvensis and ocimum sanctum were explored for endophytic actinomycetes diversity, plant growth promoting and antimicrobial activity.
Seven healthy medicinal plants from family asteraceae were selected for the isolation of endophytic fungi using standard surface sterilization techniques. Secondary metabolites of medicinal plantsderived endophytes reported in recent years are grouped according to their bioactive properties including antitumor, antimicrobial, growthpromoting, antioxidant, antithrombotic, insecticide and. Especially noted were impacts on agricultural products and livestock. Screening, isolation, biochemical and plant growth promoting. Antibacterial and antifungal potential of endophytic fungi. Apr 07, 2018 the term endophytes includes a family of microorganisms that grow intraandor intercellularly in the tissues of higher plants without any symptoms on the plants in which they reside.
Biodiversity and antimicrobial activity of endophytes. Pdf endophytic fungi residing in medicinal plants have the ability. In exchange for carbohydrate energy resources, the fungus provides benefits to the plant which can include increased water or nutrient uptake and protection from. Research article endophytic fungal diversity in medicinal plants of western ghats, india monnandasomaiahnalini, 1 ningarajusunayana, 2 andharischandrasripathyprakash 2 department of studies in botany, university of mysore, manasagangotri, mysore, karnataka, india. Antimicrobial activity of crude extracts of endophytic fungi. Jun 09, 2016 interestingly, genuine medicinal materials with the highest quality and best effects to a certain disease seems to have a special relationship with endophytic fungi.
Altogether, 75 endophytic bacterial strains were isolated from withania coagulans dunal and olea ferruginea royal, medicinal plants of karak, pakistan. Endophytic definition of endophytic by medical dictionary. A friendly relationship between endophytic fungi and. A total of 837 isolates belonging to 84 species were comprised of the stem 55. Analysis of plant growth promoting potential of endophytes. Onehundred and thirtytwo endophytic strains were isolated from 18 medicinal plants from saint katherine protectorate, egypt. Isolation of microbial endophytes from some ethnomedicinal. Over the long period of evolution, some coexisting endophytes and their host plants have established a special relationship with one and another, which can significantly influence the formation of metabolic products in plants, then affect quality and. Endophytic fungi residing in medicinal plants have the ability to produce the same or similar pharmacologically active secondary metabolites as their hosts. Antimicrobial activity of endophytic fungi isolated from.
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